Fractionation of AmV. (A) Chromatography of whole protein extract from honeybee venom using a molecular exclusion column packed with Superdex®75. The chromatographic run was carried out at a flow rate of 0.6 mL/h and monitored at 280 nm. (B) Reverse phase HPLC run yielded five main fractions designated from VI-1 to VI-5. Fraction VI-3 was confirmed as PLA2 by specific phospholipase A2 assay and fraction VI-4 as melittin after MALDI-TOFF analysis. (C) SDS-PAGE analysis of the purified proteins.