Proteic characterization of the spine extract. (A) SDS-PAGE (10%) of aqueous extract of spine. M = molecular mass standard, SE = spine extract. Arrows indicate the molecular masses. (B) Western blotting of 5, 10 and 20 μg aqueous extract of spine, incubated with anti-cathepsin B antibody. Left lane, molecular mass standard. (C) Chromatogram obtained by gel filtration of spine aqueous extract. Dashed lines indicate the enzymatic activity.