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Table 1 Kinetic parameter for the hydrolyses of FRET and fluorogenic peptides by E. lucunter aqueous extract cathepsin B/X

From: Cathepsin B/X is secreted by Echinometra lucunter sea urchin spines, a structure rich in granular cells and toxins

Substrate

KM(μM)

kcat(s-1)

kcat/KM(mM.s)-1

Z-R-MCA

147.2 ± 40.0

7.46 ± 0.60

51 ± 4

Abz-GIVR↓AK(Dnp)-OH

(63%)1

8.57 ± 0.51

0.29 ± 0.01

34 ± 2

Abz-GIVRA↓K(Dnp)-OH

(37%)

Abz-GIVRAKQ-EDDnp

ND2

ND

ND

  1. 1As determined by HPLC product peak area.
  2. 2Not determined due to poor hydrolysis.