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Table 1 Kinetic parameter for the hydrolyses of FRET and fluorogenic peptides by E. lucunter aqueous extract cathepsin B/X

From: Cathepsin B/X is secreted by Echinometra lucunter sea urchin spines, a structure rich in granular cells and toxins

Substrate KM(μM) kcat(s-1) kcat/KM(mM.s)-1
Z-R-MCA 147.2 ± 40.0 7.46 ± 0.60 51 ± 4
Abz-GIVR↓AK(Dnp)-OH (63%)1 8.57 ± 0.51 0.29 ± 0.01 34 ± 2
Abz-GIVRA↓K(Dnp)-OH (37%)
Abz-GIVRAKQ-EDDnp ND2 ND ND
  1. 1As determined by HPLC product peak area.
  2. 2Not determined due to poor hydrolysis.