Table 1 Interpretation and actions suggested after observation of different positivity combinations of the quality controls and Leishmania spp. main target in the triplex PCR reaction
Result/PCR target positivity | Interpretation/meaning | Action |
|---|---|---|
G3PD 567 bp (+) | Valid results for parasite diagnosis | Conclude diagnostic test observation |
pUC 316 bp (+) | ||
L. braziliensis 138 bp (+) | ||
G3PD 567 bp (+) | Valid results for parasite diagnosis despite small loss of DNA in extraction | Conclude diagnostic test observation |
pUC 316 bp (-) | ||
L. braziliensis 138 bp (+) | ||
G3PD 567 bp (-) | Valid results for parasite diagnosis despite small degradation of sample | Conclude diagnostic test observation |
pUC 316 bp (+) | ||
L. braziliensis 138 bp (+) | ||
G3PD 567 bp (-) | Degradation of DNA sample/presence of PCR inhibitors and/or whole DNA loss during extraction | Repeat diagnostic procedure since the blood sample collection |
pUC 316 bp (-) | ||
L. braziliensis 138 bp (-) | ||
G3PD 567 bp (-) | Degradation of DNA sample before extraction | Repeat diagnostic procedure since the blood sample collection |
pUC 316 bp (+) | ||
L. braziliensis 138 bp (-) | ||
G3PD 567 bp (+) | Significant DNA loss (affecting mainly parasite genome) during extraction process | Repeat the DNA extraction procedure |
pUC 316 bp (-) | ||
L. braziliensis 138 bp (-) | ||
G3PD 567 bp (+) | True negative result for Leishmania detection assured by the positivity of quality controls | Conclude diagnostic test observation |
pUC 316 bp (+) | ||
L. braziliensis 138 bp (-) |