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Figure 5 | Journal of Venomous Animals and Toxins including Tropical Diseases

Figure 5

From: Detection and identification of huwentoxin-IV interacting proteins by biotin-avidin chemistry combined with mass spectrometry

Figure 5

Detection of HWTX-IV interacting proteins with far-western blotting. Lane 1: without a denaturation/renaturation treatment before incubation with monobiotinylated HWTX-IV. Lane 2: with a denaturation/renaturation treatment before incubation with monobiotinylated HWTX-IV. Lane 3: incubation with HRP-avidin in the absence of monobiotinylated HWTX-IV. P: partial SDS-PAGE image showing the protein band corresponding to the far-western blotting-detected specific band on PVDF membrane. M: partial SDS-PAGE image showing protein molecular weight markers.

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