Immunoblotting and PCR assays. (A): Immunoreactivity of serum patient against H and M fractions of H. capsulatum. (B): Lane M. DNA molecular weight marker 1kb DNA ladder; Lane 1. patient serum collected in February 2008; Lane 2.- patient serum collected in June 2008; Lanes 3 to 10. negative individual sera samples, Lane Hc1. H. capsulatum strain (immunodeficiency); Lane Hc2. H. capsulatum 200 strain; Lane Pb 18. P. brasiliensis strain; Lane Pb 339 P. brasiliensis 339, Lane CN1 e CN2. C. neoformans strains (ATCC 24067) and Lane NO. negative control.