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Fig. 2 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 2

From: A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide

Fig. 2

SDS-PAGE showing the expression of pQE40HisrDHFRBa1 or pET28HisrBa1. a Gel of pQE40HisrDHFRBa1 expression. Left lane shows the molecular weight markers (MWM). Lane 1, cells with vector pQE40; lane 2, cells without IPTG induction; lane 3, cells with IPTG induction; lane 4, supernatant; lane 5, inclusion bodies; lanes 6 and 7, DHFR-Ba after affinity chromatography. b Gel of pET28HisrBa1 expression. Left lane shows the molecular weight markers (MWM). Lane 1, cells without IPTG induction; lane 2, cells with IPTG induction; lane 3, supernatant; lane 4, inclusion bodies; lanes 5, first affinity chromatography elution; lanes 6 and 7, first and second wash with 20 mM Imidazol; l 8 and 9, HisrBa1 after affinity chromatography. The protein content applied in each lane was between 5 and 10 μg

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