Fig. 3From: A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptiderpHPLC separation of oxidized pQE40HisrDHFRBa1 or pET28HisrBa1 from affinity chromatography. a The fractions from NiNta were separated employing rpHPLC (C18 column) using a gradient from 0 to 60 % B in 60 min at 1 mL/min. b The fractions from NiNta were separated using rpHPLC (C18 column) using a gradient from 0 to 50 % B in 50 min at 1 mL/minBack to article page