Fig. 5

rpHPLC separation of rBa1 from HisrDHFRBa1 and HisrBa1 using FXa. (a) Enzymatic hydrolysis of HisrDHFRBa1 using FXa where rBa1 (asterisk) was separated by rpHPLC (C₁₈ column) with a gradient from 10 to 60 % B in 50 min at 1 mL/min. (b) Enzymatic hydrolysis of HisrBa1 using FXa, rBa1 was separated by rpHPLC (C₁₈ column) with a gradient from 0 to 60 % B in 60 min at 1 mL/min. A superimposed chromatographic rpHPLC profile under the same elution conditions shows the retention time of the nBa1. The fact that rBa1 and nBa1 eluted at the same retention time suggests similar secondary and tertiary structures