Fig. 2From: Biological characterization of compounds from Rhinella schneideri poison that act on the complement systemReversed-phase FPLC of fractions S2 and S5. The C2C18 column was equilibrated with 0.1 % (V/V) trifluoroacetic acid (TFA, solution A). Adsorbed proteins were eluted using a concentration gradient from 0 to 100 % of solution B (80 % acetonitrile in 0.1 % TFA, V/V). Fractions of 0.5 mL/tube were collected at a flow rate of 0.5 mL/minBack to article page