Fig. 3

Effect of fractions S1 - S5 on the classical/lectin hemolytic pathways of complement activation. The fractions (100 μL in PBS, S1 – A₂₈₀ ~ 0.35; S2 – A₂₈₀ ~ 0.20; S3 – A₂₈₀ ~ 0.17; S4 – A₂₈₀ ~ 0.10 and S5 –A₂₈₀ ~ 0.16)) were incubated for one hour at 37 °C with normal human serum diluted 1:20 (37.5 μL) and CFD/Gel solution (12.5 μL). The positive control was run under the same conditions but in the absence of fractions. The absorbances of supernatants from cells incubated in CFD/Gel buffer (0 % lysis) and lysed in water (100 % lysis) were employed to calculate the percentage of lysis induced by NHS in the absence (positive control) or presence of the fractions (tests). The columns represent means ± SEM of an experiment performed in duplicate. ** p < 0.01, *** p < 0.001 and **** p < 0.0001 compared to the positive control