Fig. 2

Determination of molecular mass. a SDS-PAGE (10 %) under non-reducing conditions stained with Coomassie Blue R-350. b SDS-PAGE (10 %) under non-reducing conditions stained with periodic acid-Schiff (PAS) to detect glycoprotein. Hyal: hyaluronidase CdtHya1 (glycoprotein control), H7: bordonein-L. c Mass spectrum of bordonein-L obtained by MALDI-TOF (positive linear mode). d Exclusion molecular of molecular mass standards and bordonein-L (20 μg/100 μL) on a Superdex 200 10/300GL (1 × 30 cm) column equilibrated and eluted with 0.05 M sodium acetate buffer containing 0.15 M NaCl, pH 5.5, at a flow rate of 0.5 mL/min. Insert: calibration curve of the Superdex 200 10/300GL column with molecular mass standards (12,400-200,000 Da)