Fig. 2

Effect of the toxins BjussuSP-I and BpirLAAO-I on the hemolytic activity of the complement system. This figure depicts the concentration-dependent inhibitory effect of (a and c) BjussuSP-I and (b and d) BpirLAAO-I on the hemolytic activity of the (a and b) classical and (c and d) alternative pathways of the complement system. Control represents normal human serum incubated with buffer alone. Data are expressed as the mean ± standard deviation of the t^½ values obtained for each toxin concentration, based on three (CS-CP/LP) or two (CS-AP) independent experiments assayed in triplicate. *p < 0.05, **p < 0.001, or ***p < 0.0001 vs. control. BjussuSP-I: serine protease isolated from Bothrops jararacussu crude venom; BpirLAAO-I: L-amino acid oxidase isolated from Bothrops pirajai crude venom; t^½: time required to lyse 50 % of erythrocytes