Fig. 1

a Chromatographic profile of the crude venom of B. atrox on a Sephacryl S-200 molecular exclusion column. Elution was performed with 0.2 M ammonium bicarbonate buffer (AMBIC), pH 8.0, collecting 3 mL/tube at a flow rate of 20 mL/hour. b 12 % SDS-PAGE. Lanes: 1 – tube 74 (S1a), 2 – tube 82 (S1b), 3 – tube 92 (S2), 4 – tube 99 (S2 valley), 5 – molecular mass standard (260, 140, 100, 70, 50, 40, 35, 25, 15, 10 kDa), 6 – tube 103 (S3), 7 – tube 106 (S3), 8 – tube 115 (S4), 9 – tube 124 (S4), 10 – tube 145 (S5)