Fig. 4
a Chromatographic profile of fraction 6 on a C18 reverse phase column. Elution was performed using a RP-HPLC system at a flow rate of 0.5 mL/minute using a segmented concentration gradient of 0-60 % solvent B in three column volumes, 60-80 % in five column volumes and 80-100 % in one column volume. b 12 % SDS-PAGE. Lanes: 1 – PLA2, 2 – molecular mass standard (116, 66.4, 45, 35, 25, 18.4, 14.4 kDa)