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Fig. 5 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 5

From: Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A2 from Bothrops atrox snake venom

Fig. 5

a Evaluation of the purity level of fraction D4 after Vivaspin® 20 on a C18 reverse phase column. After having been subjected to ultrafiltration on a Vivaspin® 20 system, fraction D4 was evaluated by RP-HPLC using a C18 column with a linear concentration gradient of 0-100 % solvent B in five column volumes. b 12 % SDS-PAGE. Lanes: 1 – MP, 2 – molecular mass standard (260, 140, 100, 70, 50, 40, 35, 25, 15, 10 kDa)

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Journal of Venomous Animals and Toxins including Tropical Diseases

ISSN: 1678-9199

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