Fig. 6From: Purification procedure for the isolation of a P-I metalloprotease and an acidic phospholipase A2 from Bothrops atrox snake venomIsoelectric focusing of the toxins isolated from B. atrox venom on a 7Â % polyacrylamide gel. Samples: (1) isoelectric focusing standard (IEF Standards, pI ranges from 4.45 to 9.6, Bio-Rad, USA), (2) PLA2 (pI ~6.5) and (3) MP (pI ~7.4). The pI of samples was calculated from the curve of pH versus the migration distance in the gelBack to article page