Fig. 9
Evaluation of the hemorrhagic activity of the MP from B. atrox venom. Samples (n = 2) were injected intradermally into the back of BALB/c mice, and after three hours, the animals were euthanized and had their skins removed to allow the observation of the presence or absence of hemorrhagic halos. Samples (halo diameter): (a) PBS (0 mm), (b) MP 5 μg (10 mm, determined as the MHD), (c) MP 10 μg (16 mm), (d) MP 20 μg (24 mm), (e) MP 5 μg + PLA2 1:1 (12 mm), (f) MP 5 μg + 5 mM EDTA (0 mm)