Fig. 2
From: First serine protease inhibitor isolated from Rhinella schneideri poison
Inhibition assays. a. Enzyme inhibition assay of Rs20 over trypsin. b. Enzyme inhibition assay of Rs20 over chymotrypsin. c. Enzyme inhibition assay of Rs20 over elastase. In the enzyme inhibition assays, 
indicates positive control (enzyme, PBS buffer and substrate); 
indicates assay performed in the presence of Rs20 (enzyme, Rs20, PBS buffer and substrate); 
indicates negative control (denatured enzyme, PBS buffer and substrate); and 
indicates the enzymatic action of Rs20 (Rs20, buffer and substrate). The points represent the means ± SEM of one experiment performed in triplicate. ** p < 0.01; *** p < 0.001 and **** p < 0.0001 compared to the positive control