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Fig. 4 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 4

From: Cytolytic and systemic toxic effects induced by the aqueous extract of the fire coral Millepora alcicornis collected in the Mexican Caribbean and detection of two types of cytolisins

Fig. 4

a Electrophoretic profile under non-reducing (Mad) and reducing (Madr) conditions. The protein bands were visualized by the Coomassie blue staining method. b Hemolysis zymogram on a 1.5 % agar gel supplemented with 5 % egg yolk and 3 % of washed erythrocytes, two hemolytic zones were observed in the gel corresponding to ~28-30 and ~200 kDa. c PLA2 zymogram on a 2 % agarose gel supplemented with 6 % egg yolk showed only one zone with enzymatic activity corresponding to ~28-30 kDa

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