Fig. 3
From: Immunomodulatory activity of Tityus serrulatus scorpion venom on human T lymphocytes
Percentage of CD8+ T lymphocytes expressing CD25. Peripheral blood mononuclear cells (PBMC; 5 × 106cells/mL) were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE; 2.5 μM) and cultured with Tityus serrulatus scorpion venom (TsV; 25, 50, and 100 μg/mL) alone or in combination with phytohemagglutinin (PHA; 2 μg/mL) for 96 h at 37 °C, and under 5 % CO2. Then, PBMC were collected, labeled with anti-CD3/PerCP, anti-CD8/PE, and anti-CD25/APC monoclonal antibodies, and further analyzed by flow cytometry. CFSE: CFSE-stained cells cultured in RPMI; PHA: PHA-stimulated cells (positive control); 25, 50, 100: cells incubated with 25, 50, and 100 μg/mL TsV, respectively; PHA + 25, PHA + 50, PHA + 100: cells incubated with PHA plus 25, 50 and 100 μg/mL TsV, respectively. Each column represents the mean ± SEM of five experiments.*p < 0.05 vs. PHA