Fig. 2

Rechromatography of the subfractions eluted from the fractions VIIIA and VIIIB. (a) Subfraction 7. (b) Subfraction 9. The C18 column (2.1 mm × 250.0 mm, 3.6 μm particles, Phenomenex) was equilibrated with 0.1 % TFA and the proteins were eluted using a concentration gradient from 0 to 100 % of solution B (80 % ACN in 0.1 % TFA), represented by the dashed line. Absorbance was monitored at 214 nm, at 25 °C, using a FPLC Äkta Purifier UPC-10 system. Fractions of 0.3 mL/tube were collected at a flow rate of 0.4 mL/min