Table 1 N-terminal sequence of the main peaks eluted from the chromatographic steps. Assignment of the peaks to protein families by BLAST against a Tityus venom database
Peak | N-terminal sequence | Protein family (Uniprot ID) |
|---|---|---|
6 | KDKMKAGWERLTSQSEYACP… | Ts19 Frag-II (P86822) |
KIKEKIIEAKDKMKAGWERL… | Ts19 Frag-I (P86822) | |
7 | KKDGYPVEYDNCAYICWNYDNAY… | Ts3 (P01496) |
KDKMKAGWERLTSQSEYACPAID… | Ts19 Frag-II (P86822) | |
8 | KIKEKIIEAKDKMKAGWERLTSQSEYACPAIDKFCEDHCAAKKAVGKCDDFKCNCIK | Ts19 Frag-I (P86822) |
KEGYAMDHEGCKFSCFIRPAGFCDGYCKTHLKASSGYCAWPACYCYGVPD… | Ts2 (P68410) | |
9 | KIKEKIIEAKDKMKAGWERLTSQSEYA… | Ts19 Frag-I (P86822) |
KKDGYPVEYDNCAYICWNYDNAYCDKL… | Ts3 (P01496) | |
KEGYAMDHEGCKFSCFIRPAGFCDGYC… | Ts2 (P68410) | |
10 | GREGYPADSKGCKITCFLTAAGYCNTECTL… | Ts4 (P45669) |
11 | GREGYPADSKGCKITCFLTA… | Ts4 (P45669) |
12 | GREGY… | Ts4 (P45669) |
13 | KLVALIPNDQLRSILKAVVHKVAKTQFGCPAYEGYCNDHCNDIERKDG… | Ts8 (P69940) |
GLREKHVQKLVALIPNDQLRSILKAVVHKVAKTQFGCPAYEGYCNDHC… | Ts8 propeptide (P69940) | |
GREGYPADSKG… | Ts4 (P45669) | |
9.2 | KIKEKIIEAK… | Ts19 Frag-I (P86822) |
KEGYAMDHEG… | Ts2 (P68410) | |
9.3 | KIKEKIIEAKDKMKA… | Ts19 Frag-I (P86822) |
KEGYAMDHEGCKFSC… | Ts2 (P68410) |