Indian scorpions collected in Karnataka: maintenance in captivity, venom extraction and toxicity studies

Table 2 LD₅₀ of H. rugiscutis venom injected to mice subcutaneously (n = 5) and calculated by graphical method of Miller and Tainter

Group	Dose (mg/kg bw)	Log of dose	Volume of injection (mL)	Mice (death/total)	Percentage of death (%)	Corrected percentage	Probit value
1.	2.60	0.414	0.2	0/5	0	5^a	3.36
2.	2.80	0.447	0.2	1/5	20	20	4.16
3.	3.02	0.480	0.2	3/5	60	60	5.25
4.	3.26	0.513	0.2	4/5	80	80	5.84
5.	3.52	0.544	0.2	5/5	100	100^a	6.64

^aCorrection formula: \( 0\%=100\left(\frac{0.25}{n}\right)100\%=100\left(n-0.25/5\right) \); bw: body weight

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