Fig. 4

Determination of IL-10 concentration (pg.mL⁻¹) in the supernatant of uninfected macrophages (n) and infected macrophages (i) after melittin treatment (0, 0.7, 1.47 and 2.5 μg.mL⁻¹) at 24, 48 and 72 h. Data are presented as mean of three independent measurements. ● p < 0.05, for 48 and 72 h treatment with 2.5 μg.mL⁻¹ melittin versus non-infected cells. ∆ p < 0.05 for 24 h treatment with 2.5 μg.mL⁻¹ melittin versus non-infected non-treated cells. All treated infected cells (24, 48 and 72 h; 0, 0.7, 1.47 and 2.5 μg.mL⁻¹) were statistically different (p < 0.05) of the corresponding non-treated cells