Fig. 5

Determination of NO levels in the supernatant of uninfected macrophages (n) and infected macrophages (i) after melittin treatment (0, 0.7, 1.47 and 2.5 μg.mL⁻¹) at 24, 48 and 72 h. Data are presented as mean of three independent measurements.* p < 0.05 for infected cells versus non-infected cells (24, 48 and 72 h) without melittin addition. All treated infected cells (24, 48 and 72 h; 0, 0.7, 1.47 and 2.5 μg.mL⁻¹) are statistically different (p < 0.05) of the corresponding non-treated cells