Fig. 3
Purity assessment of synthetic purified peptide LyeTx I derivatives modified with the chelating agent HYNIC, by RP-HPLC (Ettan LC, GE HealthCare, USA). Chromatograms of synthetic purified HYNIC-LyeTx I (gray line) and LyeTx I-K-HYNIC (black line): PepMap C18TM column (5 μm, 150.0 mm × 4.6 mm) equilibrated with 0.1 % (v:v) TFA in water (eluent A) and eluted by a linear gradient of 0.1 % (v:v) TFA in acetonitrile (eluent B); the flow was 1.0 mL.min−1 and the detection was at 214 nm. PP: peak of peptide