Fig. 4

LyeTx I-K-HYNIC-^99mTc evaluation by RP-HPLC (717 Plus Autosampler, Waters, USA) associated with radioactivity determination of the collected fractions by automatic gamma counter (Wizard, Finland). a Chromatograms of EDDA (red line), tricine (green line) and LyeTx I-K-HYNIC (black line): ACE 5 C8 column (5 μm, 250.0 mm × 4.6 mm) equilibrated with 0.1 % (v:v) TFA in water (eluent A) and eluted by a linear gradient of 0.1 % (v:v) TFA in acetonitrile (eluent B); the flow was 1.0 mL^.min⁻¹ and the detection was at 214 nm. b Radiochromatogram of LyeTx I-K-HYNIC-^99mTc: ACE 5 C8 column (5 μm, 250.0 mm × 4.6 mm) equilibrated with 0.1 % (v:v) TFA in water (eluent A) and eluted by a linear gradient of 0.1 % (v:v) TFA in acetonitrile (eluent B); the flow was 1.0 mL^.min⁻¹. cpm: counts per minute