Table 1 Solvent conditions for RP-HPLC
(A) Crude synthetic product purification | (B) Purified synthetic product analysis | (C) LyeTx I-K-HYNIC-99mTc evaluation | |||
|---|---|---|---|---|---|
Time | Gradient of eluent B | Time | Gradient of eluent B | Time | Gradient of eluent B |
(min) | (%) | (min) | (%) | (min) | (%) |
0–8.2 | 0 | 0–3.7 | 0 | 0–5.0 | 0 |
8.2–12.4 | 0–30 | 3.7–33.5 | 0–100 | 5.0–30.0 | 0–55 |
12.4–50.0 | 30–55 | 33.5–39 | 100 | 30.0–35.0 | 55–100 |
50.0–54.0 | 55–100 | 35.0–45.0 | 100 | ||
54.0–62.5 | 100 | ||||