Fig. 5

Immunolocalization of Hlsv in isolated mouse cardiomyocytes. a Localization of Hlsv detected with anti-Hlsv IgGs in ventricular myocytes. Cells were treated with Hlsv, incubated with anti-Hlsv IgGs antibodies, followed by anti-rabbit IgG conjugated to Alexa Fluor 488 (Invitrogen, USA). As control, cardiomyocytes were treated only with secondary antibodies (control 1) or with IgG anti-Hlsv + secondary antibodies (control 2). Seven cells were analyzed in each group. b Bar graph shows the concentration-dependency of venom binding to cardiac cells