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Fig. 1 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 1

From: Heterologous expression, protein folding and antibody recognition of a neurotoxin from the Mexican coral snake Micrurus laticorallis

Fig. 1

Gene construction of the plasmid carrying HisrMlat1. a The nucleotide and amino acid sequence of HisrMlat1. b The nucleotide and amino acid sequence of HisrMlat1 after modification of the Arg N-terminal. c Scheme of the expression vector pQE30XaHisrMlat1. The His-tag is part of the 5′-nucleotide sequence of pQE30, in such a manner that the sequence to be expressed is the one shown, in which the cleavage site for the restriction enzyme is indicated (BamHI and PstI). d Amino acid sequence of HisrMlat1. The His-tag is in bold, the FXa cleavage site is in italics and the modification of the residue Lys is in bold

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