Table 1 Primer design of gene-specific primers and PCR product size
Forward primer | Reverse primer | Product size (bp) |
|---|---|---|
Full nucleotide sequence active form | ||
F4 GCCAGACTTTTCATGGAGGA (GSP1 for active) | R3 (7) ATCAGGGGTCAGTTCACGTC (GSP1 for active) | 225 |
Adaptor primer (AP) 5′GGCCACGCGTCGACTAGTAC (T) 16 (GSP for cDNA synthesis of 3′ RACE system) | R4 (8) CGTCGGTCTCGGTAAGAAAA |  |
Abridged universal amplification primer (AUAP) | R5 (9) GTTCTCGTGCATCGCTGTAA | Â |
VesT2a (F) NcoI CCATGGCTTCCGAGAGACC | VesT2a (R) XhoI CTCGAGTTAGTTAACGGCTTCTG | Â |
Full nucleotide sequence inactive form | ||
F1 CGAAAAGGAAGCGTCGAATA (GSP for RT-PCR inactive form) | R1 CATCTTGTCGTTCTCGCTCA (GSP for RT-PCR inactive form) | 190 |
F2 CTTCGGCGTCTATTTCAAGG (GSP for RT-PCR inactive form) | R2CCGCTAAGACAGTGGGGATA (GSP for inactive form) | 229 |
Adaptor primer (AP) 5′GGCCACGCGTCGACTAGTAC (T) 16 (GSP for cDNA synthesis of 3′ RACE system) | R2 (1) CATCTTGTCGTTCTCGCTCA (GSP for RT-PCR inactive form) |  |
Abridged universal amplification primer (AUAP) | R1 (2) CCGCTAAGACAGTGGGGATA (GSP for inactive form) | Â |