Fig. 5
Effect of F3 fraction on the percentage of DNA fragmentation and caspase-3 activity. a Effect of F3 fraction on percentage of DNA fragmentation in NCI-H358 cells. b Caspase-3 activity in NCI-H358 cells was analyzed using CASP-3-C colorimetric assay kit. Cells were treated with F3 fraction (½ IC50, IC50 and 2 IC50) for 24 h with (black bars) or without (grey bars) caspase-3 inhibitor Ac-DEVD-CHO. The results are expressed as the mean ± SD of triplicate determinations, representative of three independent experiments. Significances indicated were in comparison to control (*p < 0.05; **p < 0.01; ***p < 0.001 vs. control)