Fig. 3

Effect of a L-selectin binder on MT-II- induced articular hyperalgesia. MT-II (10 μg/joint) or PBS (vehicle) was injected in tibio-tarsal articulation (25 μL). Fucoidan (fuco), a L-selectin binder (5 mg/kg, i.v.) or saline (vehicle) was injected 15 min prior to MT-II. a Pain threshold was determined using a modified electronic pressure-meter test 8 h after MT-II injection, and represented as force (in g). b The neutrophil migration to the tibio-tarsal joint region of mice was evaluated by the myeloperoxidase (MPO) kinetic-colorimetric assay, tested 8 h after MT-II injection. Each point represents the mean ± SEM of six animals. *p < 0.05 and ***p < 0.001 indicate statistically significant differences when compared with control group (vehicle + vehicle). #p < 0.05 and ###p < 0.001 indicate statistically significant differences when compared with MT-II group (MT-II + vehicle)