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Table 1 Myotoxin-induced cell migration to the joint

From: Articular inflammation induced by an enzymatically-inactive Lys49 phospholipase A2: activation of endogenous phospholipases contributes to the pronociceptive effect

Treatment Groups (n = 6) Total cells (× 106/mL) Mononuclear cells (× 106/mL) PMN cells (× 106/mL)
   1 h 4 h 8 h 12 h 8 h 8 h
PBS 6 2.12 ± 0.70 3.72 ± 1.80 1.09 ± 0.61 0.24 ± 0.14 0.25 ± 0.13 1.65 ± 0.77
Myotoxin 5 μg/joint 6 2.07 ± 0.6 83.44 ± 25.2 52.27 ± 23.67 5.20 ± 1.54 7.97 ± 4.65 44.30 ± 19.07
Myotoxin 10 μg/joint 6 18.71 ± 4.10 73.16 ± 22.10 163.08 ± 48.04a 28.12 ± 7.57 8.15 ± 2.87 154.93 ± 34.16a
Myotoxin 15 μg/joint 6 6.12 ± 1.80 48.5 ± 22.70 66.38 ± 21.50 21.32 ± 8.38 3.91 ± 0.86 62.47 ± 13.73
Myotoxin 20 μg/joint 6 10.90 ± 12.75 41.37 ± 20.10 43.15 ± 5.53 12.75 ± 3.40 2.79 ± 0.85 40.36 ± 4.88
Carrageenin 6 4.74 ± 1.10 49.43 ± 7.20 109.14 ± 30.31a 15.64 ± 3.65 7.21 ± 2.36 101.93 ± 28.22a
BSA 6 0.50 ± 0.30 1.00 ± 0.5 1.50 ± 3.40 2.00 ± 0.50 / /
  1. Total and differential cellular influx to the femoral-tibial-patellar articulation, evaluated 1, 4, 8 and 12 h after myotoxin II (5, 10, 15 and 20 μg/joint) injection. Total and differential cell counts were performed using a Neubauer chamber (1:20 dilution v:v) and stained smears (violet crystal 0.5%), respectively. A total of 100 cells were counted on a light microscope
  2. aSignificantly different from mean values of control group (BSA)