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Table 2 Evaluation of histamine, serotonin, nitric oxide and metalloproteinases in the myotoxin-induced hypernociceptive effect

From: Articular inflammation induced by an enzymatically-inactive Lys49 phospholipase A2: activation of endogenous phospholipases contributes to the pronociceptive effect

Treatment Force in grams evaluated 8 h after myotoxin injection
Saline + PBS 49.90 ± 1.72
DMSO + PBS 49.60 ± 2.11
Saline + Myotoxin 25.58 ± 0.59a
DMSO + Myotoxin 23.64 ± 0.79a/NS
Zyleuton + Myotoxin 29.82 ± 3.03a/NS
Methysergide + Myotoxin 26.72 ± 0.85a/NS
Promethazine + Myotoxin 24.74 ± 1.04a/NS
L-NMMA + Myotoxin 29.14 ± 1.72a/NS
GM6001 + Myotoxin 26.06 ± 0.69a/NS
Methysergide + PBS 49.82 ± 1.31
Promethazine + PBS 50.06 ± 2.18
L-NMMA + PBS 48.26 ± 3.87
GM6001 + PBS 44.54 ± 2.33
  1. Articular hyperalgesia induced by MT-II in rats in the presence or in the absence of different pharmacological treatments. The articular hypernociception was determined by a dorsal flexion of the tibio-tarsal joint using a modified electronic pressure-meter test and was represented as force (in g), observed 8 h after MT-II injection. Zyleuton: 5-lipoxygenase inhibitor; methysergide: antagonist of H1 histaminergic receptor; promethazine: antagonist of serotoninergic receptors; L-NMMA: inhibitor of nitric oxide synthase; GM6001: a potent broad-spectrum hydroxamate inhibitor of matrix metalloproteinases (inhibitor of 1-, 2-, 3-, 8- and 9-MMPs)
  2. NS Not significantly different from mean values of myotoxin group
  3. aSignificantly different from mean values of control group (Saline or DMSO + PBS)