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Table 2 Evaluation of histamine, serotonin, nitric oxide and metalloproteinases in the myotoxin-induced hypernociceptive effect

From: Articular inflammation induced by an enzymatically-inactive Lys49 phospholipase A2: activation of endogenous phospholipases contributes to the pronociceptive effect

Treatment

Force in grams evaluated 8 h after myotoxin injection

Saline + PBS

49.90 ± 1.72

DMSO + PBS

49.60 ± 2.11

Saline + Myotoxin

25.58 ± 0.59a

DMSO + Myotoxin

23.64 ± 0.79a/NS

Zyleuton + Myotoxin

29.82 ± 3.03a/NS

Methysergide + Myotoxin

26.72 ± 0.85a/NS

Promethazine + Myotoxin

24.74 ± 1.04a/NS

L-NMMA + Myotoxin

29.14 ± 1.72a/NS

GM6001 + Myotoxin

26.06 ± 0.69a/NS

Methysergide + PBS

49.82 ± 1.31

Promethazine + PBS

50.06 ± 2.18

L-NMMA + PBS

48.26 ± 3.87

GM6001 + PBS

44.54 ± 2.33

  1. Articular hyperalgesia induced by MT-II in rats in the presence or in the absence of different pharmacological treatments. The articular hypernociception was determined by a dorsal flexion of the tibio-tarsal joint using a modified electronic pressure-meter test and was represented as force (in g), observed 8 h after MT-II injection. Zyleuton: 5-lipoxygenase inhibitor; methysergide: antagonist of H1 histaminergic receptor; promethazine: antagonist of serotoninergic receptors; L-NMMA: inhibitor of nitric oxide synthase; GM6001: a potent broad-spectrum hydroxamate inhibitor of matrix metalloproteinases (inhibitor of 1-, 2-, 3-, 8- and 9-MMPs)
  2. NS Not significantly different from mean values of myotoxin group
  3. aSignificantly different from mean values of control group (Saline or DMSO + PBS)