Fig 7

Neutralization of edema-forming activity of D. acutus venom by IgY (mean ± SD, n = 4). Mixtures containing a constant amount of venom and various dilutions of IgY were incubated at 37 °C for 30 min. Afterwards, aliquots of the mixtures (20 μL containing three MED of venom) were injected into the right footpad of mice, whereas the left foot pad received 20 μL of physiological saline. The weight of both feet was estimated 3 h after injection with an electronic scale and edema was expressed as the percentage increment in weight of the right footpad when compared to the left one