Fig. 2
From: Rattlesnake Crotalus molossus nigrescens venom induces oxidative stress on human erythrocytes
PAGE of hemoglobin under (a) non-denaturing and (b) denaturing conditions – erythrocytes incubated with CMNv. Erythrocytes treated with different concentrations of CMNv for 24 h were centrifuged and soluble hemoglobin was used in this experiment. Lanes 1–8 show the erythrocytes treated with different venom concentrations, lane 9 shows 50 μg of CMNv, lanes 10 and 11 show Oxy-Hb and Met-Hb controls, respectively. The hemoglobin (64 kDa) is marked with a black arrow, α-globin and β-globin (16 kDa) are marked with a red arrow head