Skip to main content
Fig. 2 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 2

From: Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine

Fig. 2

Analyses of Crotalus durissus terrificus venoms by RP-HPLC. The venoms of C. d. terrificus (batches 2014CDU00301 and 2014CDU00201) were pooled from (a) crotamine-positive specimens collected in southern and (b) crotamine-negative specimens collected in southeastern Brazil, respectively. The pooled venom for immunization was obtained by combining (c) the same ratio of crotamine-positive and crotamine-negative venoms. The elution conditions were: isocratic gradient with 5% of 0.1% TFA in 100% of acetonitrile (solvent B for 10 min followed by 5–15% B for 20 min, 15–45% B for 120 min and 45–70% B for 20 min, with a final isocratic step of 70% B for 5 min at a flow rate of 1 mL/min. Protein families associated with HPLC peaks were: 1 – crotamine, 2 – disintegrin, 3 – crotoxin acid chain, 4 to 7 – crotoxin basic chain, and 8 – fractions of low expression toxins including D49-PLA2, serine protease (gyroxin), C-type lectin (convulxin) and PIII-metalloproteases

Back to article page