Fig. 3

HPLC analysis of the crude venom. Column: COSMOSIL 5C18-AR-300, 4.6 mm × 150 mm (Nacalai Tesque). Eluent: (I) CH₃CN containing 0.1% TFA and (II) H₂O containing 0.1% TFA. Elution: linear gradient from (I):(II) = 0.1:99.9 to 60:40. Flow rate, 1.0 mL/min for 45 min. Detection: UV 210 nm. Purity of each fraction was monitored by MALDI-TOF MS. A: m/z 2066; B and C: mixture (m/z 2066 was mainly detected); D: m/z 16508 (PLA₂ homolog); E: mixture; F: m/z 1939.3 (Xac-1); G: m/z 1900.3 (Xac-2); H: mixture