Fig. 3From: Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondriaEffect of ergosterol in the mitochondrial function of trypomastigotes. Fluorescence of Mitotracker Red CM-H2XROS dye (500 nM) was spectrofluorimetrically measured after 60 min of incubation. Maximal and minimal fluorescence was achieved by non- or FCCP (10 μM) treatments, respectively. Fluorescence was quantified by calculating the mean percentage of untreated parasites (100%). *p < 0.001. A representative assay is shownBack to article page