Fig. 3

The catalytic activity of NnV on several chromogenic substrates. a NnV was preincubated with different substrates [N-Succinyl-Ala-Ala-Ala-ρNA (for elastase), Nα-Benzoyl-DL-Arg-ρNA (for trypsin), N-(p-Tosyl)-Gly-Pro-Lys-ρNA·acetate salt (for plasmin), N-Benzoyl-Phe-Val-Arg-ρNA·HCl (for thrombin), N-Succinyl-Ala-Ala-Pro-Phe-ρNA (for chymotrypsin)], 0.5 mM for 1 h. The catalytic activity of NnV was assayed at 405 nm. b NnV was preincubated with different protease inhibitors (1 mM), followed by reacting chymotrypsin substrate for 30 min. Data represent as mean ± SD from the three fields *p < 0.05 and **p < 0.01, compared to control