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Fig. 5 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 5

From: In vitro characterization of jellyfish venom fibrin(ogen)olytic enzymes from Nemopilema nomurai

Fig. 5

The purification of serine protease from NnV. a Crude venom was dissolved in 10 mM Tris-HCl (pH 7.8) buffer and centrifuged for 30 min at 13000 × g. The supernatant was loaded on a DEAE column and the proteins were eluted with a NaCl linear gradient of 0 to 80% at flow rate of 1 mL/min. b and d The fibrinolytic activity and protein profile of each fraction were evaluated by by zymography and SDS-PAGE. c The fibrinolytic activity was inhibited by PMSF 1 mM, but not by EDTA

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