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Fig. 3 | Journal of Venomous Animals and Toxins including Tropical Diseases

Fig. 3

From: Preparation of monoclonal antibodies against gamma-type phospholipase A2 inhibitors and immunodetection of these proteins in snake blood

Fig. 3

Western blot analysis of the culture supernatant of 18 IgG positive hybridoma clones. a Immunoblotting of 18 mAbs to identify recombinant His6-PLIγ. The first lane was blotted with anti-His tagged antibody as positive control, while others were the 18 IgG positive hybridoma culture supernatant. The same position of all lanes indicated that all the 18 mAbs reacted with recombinant S. annularis PLIγ. b Immunoblotting of the 18 mAbs to identify native S. annularis serum. The positive bands in all lanes indicated the availability of the 18 mAbs in natural PLIγ. All the natural PLIγ showed two close bands by the immunoblotting, which indicated natural PLIγ is composed of two subunits

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Journal of Venomous Animals and Toxins including Tropical Diseases

ISSN: 1678-9199

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