Table 1 Primer sequences
Gene | Primer sequences |
|---|---|
IFN-γ | F 5’- AgA ggA Tgg TTT gCA TCT ggg TCA-3’ R 5’- ACA ACg CTA TgC AgC TTg TTC gTg-3’ |
IL-17 | F 5’- ACC gCA ATg AAg ACC CTg AT-3’ R 5’- TCC CTC CgC ATT gAC ACA-3’ |
IL-10 | F 5’- gCC AAg CCT TAT Cgg AAA Tg-3’ R 5’- CAC CCA ggg AAT TCA AAT gC-3´ |
TGF-β | F 5’ - AAC AAT TCC Tgg CgT TAC CTT – 3’ R 5’ - CTg CCg TAC AAC TCC AgT gA- 3’ |
Foxp3 | F 5’- CCC Agg AAA GAC AGC AAC CTT −3’ R 5’- TTC TCA CCA CCA ggC CAC TTg −3’ |
GAPDH | F 5’ - CCT CgT CCC gTA gAC AAA ATg-3’ R 5’ - TgA Agg ggT CgT TgAT ggC-3’ |
T. cruzi | F- 5’-gCT CTT gCC CAC AMg ggT gC-3’ R- 5’-CCA AgC AgC ggA TAg TTC Agg-3’. |