Table 1 Full sequence of ponericin W5 (P82427) and its fragments. The full peptide corresponding to ponericin W5 and its fragments were identified in the venom of N. villosa. Isoforms of the full peptide and its fragments were also identified
Sequence | Condition | Mw (Da) | Peptide processing/Mutations |
|---|---|---|---|
FWGALIKGAAKLIPSVVGLFKKKQ | Ponericin W5 | 2598.57 | – |
FWGALIKGAAKLIPSVVGLFKKKQ | Nv1; Nv2; Nv3; Nv4 | 2598.57 | – |
FWGALIKGAAKLIPSVVGLFKKK | Nv1;Nv3; Nv4 | 2470.51 | Carboxypeptidase |
FWGALIKGAAKLIPSVVGLFKK | Nv1 | 2342.42 | Carboxypeptidase |
FWGALIKGAAKLIPSVVGLF | Nv4 | 2086.23 | Carboxypeptidase |
FWGALIKGAAKLIPSVVGL | Nv2;Nv4 | 1939.16 | Carboxypeptidase |
FWGALIKGAAKLIPSVVG | Nv1; Nv3; Nv4 | 1826.08 | Carboxypeptidase |
FWGALIKGAAKLIPSVV | Nv1 | 1769.06 | Carboxypeptidase |
ALIKGAAKLIPSVVGLFKK | Nv4 | 1952.25 | Endopeptidase |
ALIKGAAKLIPSVVGLF | Nv1 | 1696.06 | Endopeptidase |
ALIKGAAKLIPSVVG | Nv4 | 1548.99 | Endopeptidase |
GAAKLIPSVVGLFKK | Nv1 | 1526.95 | Endopeptidase |
GAAKLIPSVVGLF | Nv4 | 1270.76 | Endopeptidase |
AAKLIPSVVGLF | Nv1; | 1213.74 | Endopeptidase |
IPSVVGLFKK | Nv4 | 1342.83 | Endopeptidase |
PSVVGLFKKK | Nv3; | 1101.69 | Endopeptidase |
IPSVVGLFK | Nv4 | 958.58 | Endopeptidase |
WGALIKGAAKLIPSVVGLFKKKQ | Nv1; | 2451.50 | Aminopeptidase |
GALIKGAAKLIPSVVGLFKKKQ | Nv4 | 2265.42 | Aminopeptidase |
ALIKGAAKLIPSVVGLFKKKQ | Nv1;Nv4 | 2208.40 | Aminopeptidase |
IKGAAKLIPSVVGLFKKKQ | Nv1; Nv4 | 2024.28 | Aminopeptidase |
GAAKLIPSVVGLFKKKQ | Nv1; Nv2;Nv4 | 1783.10 | Aminopeptidase |
AAKLIPSVVGLFKKKQ | Nv1;Nv2; Nv4 | 1726.08 | Aminopeptidase |
PSVVGLFKKKQ | Nv1; Nv2; Nv4 | 1229.74 | Aminopeptidase |
FWGALIKGAAKLIPSVVGMFKKKQ | Nv1; Nv3; Nv4 | 2616.53 | (Leu19Met) |
FWGALIKGAAKLIPSVVGM | Nv3 | 1957.12 | (Leu19Met)/carboxypeptidase |
GALIKGAAKLIPSVVGMFKKKQ | Nv3 | 2283.38 | (Leu19Met)/aminopeptidase |
ALIKGAAKLIPSVVGMFKKKQ | Nv3 | 2226.36 | (Leu19Met)/aminopeptidase |
ALIKGAAKLIPSVVGM | Nv3 | 1566.95 | (Leu19Met)/endopeptidase |
GAAKLIPSVVGMFKKKQ | Nv1;Nv3 | 1801.06 | (Leu19Met)/endopeptidase |
FW*GALIKGAAKLIPSVVGLFKKKQ | Nv2; Nv4 | 2614.57 | *Oxidation |
FW*GALIKGAAKLIPSVVGL | Nv2 | 1955.16 | *Oxidation/carboxypeptidase |
FW*GALIKGAAK | Nv3 | 1176.66 | *Oxidation/carboxypeptidase |