Fig. 2

Two-dimensional electrophoresis and mass spectrum of PocTX. a PocTX (15 μg) was analyzed by 2D SDS-PAGE electrophoresis using a 7-cm strip, with non-linear pH values from 3 to 10. After isoelectric focusing, the strip was applied to a 15% polyacrylamide gel. The gel was stained with Coomassie Blue G250. b Then, PocTX was analyzed by mass spectrometry (MALDI-TOF) to determine its m/z ratio, using sinapinic acid as the ionization matrix, in the mass range of 5000-60,000 m/z