Fig. 2

ALT-C stimulates the transmigration of MDA-MB-231 cells through a monolayer of HMEC-1 cells. a MDA-MB-231 cells were plated in wells containing HMEC-1 cells, and 5% of fetal bovine serum (FBS) was used as a chemoattractant in the lower chamber. Tumor cells were first stained with PKH26 Red Fluorescent Cell Linker and after 16 h of transmigration assay the cells were fixed, stained with DAPI and counted (an average of eight fields from each treatment). Negative control means the assay in the absence of FBS in the lower chamber. The assay was performed in triplicate with two independent assays (n = 2). The results were compared using ANOVA followed by Tukey’s test (* p < 0.05, and *** p < 0.001). b Representative images of transmigrated cells of each treatment. Bar represents 50 μm