Fig. 3

ALT-C effects on: (a) MDA-MB-231 cells; (b) fibroblasts; and (c) HMEC-1 cells were plotted as a percentage of wound closure 24 h after wounding. FBS 10% represents cells in presence of medium with FBS (10%) as positive control. The assay was performed in triplicate with two independent assays (n = 2). p values were determined using ANOVA followed by Tukey’s test, considering significant when p < 0.05. Representative photos of wounds were taken at time zero and 24 h after wounding. Cells were stained with crystal violet 0.1%