Fig. 6

ALT-C induces FAK phosphorylation at 10 nM but not at 100 and 1000 nM. a FAK and p-FAK protein level revealed by Western blotting in lysates extracted from MDA-MB-231 treated with ALT-C (10, 100 and 1000 nM) and control (0 nM). b Values represent the normalized densitometry ratio of p-FAK and FAK and the p value was determined using ANOVA followed by Tukey’s test. Values were also previously normalized to the level of GAPDH densitometry. ** p < 0.01 compared to control (0 nM). The assay was performed in independent assays (n = 2) and Western blotting in quadruplicate